http://kidneyfunction.org Renal Information Sun, 22 Jan 2012 23:48:20 +0000 en hourly 1 http://wordpress.org/?v=3.0.2 http://kidneyfunction.org/analytical-and-biological-variation-in-measures-of-anemia-and-iron-status-in-patients-treated-with-maintenance-hemodialysis-corrected-proof/ http://kidneyfunction.org/analytical-and-biological-variation-in-measures-of-anemia-and-iron-status-in-patients-treated-with-maintenance-hemodialysis-corrected-proof/#comments Tue, 20 Jul 2010 05:00:00 +0000 David B. Van Wyck, Harry Alcorn, Resmi Gupta http://10.1053/j.ajkd.2010.05.009 Background: To make informed decisions in dosing erythropoiesis-stimulating agents and intravenous iron therapy, clinicians must determine whether differences between current and previous test results for anemia and iron status markers reflect expected variation, a significant change, or an actual trend.Study Design: Prospective observational cohort study.Setting & Participants: 30 patients undergoing thrice-weekly in-center hemodialysis.Predictor: Within-patient biological variations in hemoglobin (Hb) level, hematocrit (Hct), reticulocyte Hb content, transferrin saturation (TSAT), and ferritin level were determined over 12 consecutive treatment days.Outcomes & Measurements: We separately measured same-sample analytical variation and within-patient biological variation (coefficient of variation), then calculated the number of sampling days needed to determine the true or homeostatic value for each analyte with 95% probability. We also evaluated whether results differed among the first, second, and third dialysis days of the week.Results: Biological variation differed by analyte. Hb level (4.0%), Hct (4.0%), and reticulocyte Hb content (4.8%) showed much lower variation than TSAT (38.2%) or ferritin level (15.1%). Analytical variation ranged from 2.0%-6.9% for all analytes. We found that one sample day would be sufficient to establish the true mean Hb level or Hct within a level of closeness ±20% and 95% probability. For the same levels of closeness and probability, one sample day would be needed for reticulocyte Hb content, 15 for TSAT, and 3 for ferritin level. No pairwise comparison for any of the 5 analytes yielded a significant difference between results obtained on the first, second, or third dialysis day of the week.Limitations: These findings may not apply to other patient populations.Conclusions: Low biological variation renders Hb level, Hct, and reticulocyte Hb content, but not TSAT and ferritin level, suitable for trend analysis using results from 2 successive samples. TSAT and ferritin test results, unlike reticulocyte Hb content, have limited value in evaluating changes in iron status within individual hemodialysis patients.

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